Eppendorf BioSpectrometer® basic

Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
. Eppendorf BioSpectrometer® basic

The Eppendorf BioSpectrometer® basic makes your work easier with the following features: simplest handling, guided software procedures, operation directly on the device, preprogrammed methods, automatic evaluation of measurement data and clear presentation of results. More information

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Product Information

Building on the legacy of the Eppendorf BioPhotometer D30, the Eppendorf BioSpectrometer basic shares its simplicity, flexibility and reliability. In addition to the Biophotometer D30, the Biospectrometer basic has freely selectable wavelengths from 200  –  830 nm (selectable in 1 nm increments). This allows the user to define the parameters of each assay, whether it be a scan, a single-wavelength measurement or a multi-wavelength measurement. The Eppendorf BioSpectrometer basic caters to more disciplines as the wavelengths are not pre-defined and allows for non-routine assays or new assays to be easily programmed (in addition to the pre-programmed routine methods). The Eppendorf BioSpectrometer basic is a robust yet flexible ‘workhorse’.

Features

  • Absorbance measurement for one or more wavelengths, recording of wavelength scans from 200 nm to 830 nm (increment: 1 nm)
  • Automatic evaluation and storage of measurement data with clear presentation of results
  • Spectral graph to display sample purity with automatic ratio calculation
  • Freely programmable applications with evaluation via factor, standard or standard series and freely programmable parameters
  • Two-wavelength method with substraction or division evaluation
  • Integrated application and results memory
  • Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
  • Guided software process to minimize errors
  • Integrated self-test and calibration history

Applications

  • Nucleic acid quantification
  • Direct protein quantification (UV 280 nm)
  • Microvolume measurement via µCuvette G1.0 for highly concentrated samples
  • Bacteria growth measurement (OD 600)
  • Colorimetric assays for protein quantification, e.g., BCA, Bradford, Lowry
  • Evaluation of FOI for dye-labeled biomolecules (nucleic acid or proteins)
  • Free selectable wavelengths, e.g., 340 nm: assays using NADPH or NAPH, 405 nm: assays using para-nitrophenol, 420 nm: assays using ortho-nitrophenol, 490 nm: colorimetric fructose determination, 490 nm: cytoxicity endpoint assay
 BioSpectrometer® basic
Absorption measuring range0 A – 3.0 A (260 nm)
BSA concentration range (UV 280 nm) µCuvette® G1.0758 ng/µL -45,450 ng/µL
±30 ng/µL at A = 0
±76 ng/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette® 10 mm75 ng/µL - 4,545 ng/µL
±3 ng/µL at A = 0
±7 ng/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette® 2 mm379 ng/µL - 22,725 ng/µL
±15 ng/µL at A = 0
±38 ng/µL at A = 1 (0.5 %)
BSA concentration range (for 1 to 10 mm path length)76 ng/µL - 45,450 ng/µL
(The factor for BSA is 1,515)
Cuvette shaft12.5 mm × 12.5 mm
Detector TypeCMOS photodiode array, 1024 pixel
Emission wavelength I fluorescence
Emission wavelength II fluorescence
Excitation wavelength fluorescence
Fluorescence measuring range
Light source absorptionXenon flash lamp
Light source fluorescence
Measuring principle absorptionSingle-beam absorption spectrophotometer with reference beam
Measuring principle fluorescence
Method memory>100 method programs
Operator guidance languageSpanish, Italian, French, English, German, Japanese
Power consumptionApprox. 15 W during operating step
Approx. 5 W with dimmed display
Random error absorption≤0.002 at A = 0
≤0.005 (0.5 %) at A = 1
Random error fluorescence
Random error wavelength±1 nm
Random temperature error
Sample light path length8.5 mm
Scanningyes
Smallest step size1 nm
Spectral bandwidth≤4 nm
Systematic error absorption±1 % (A = 1)
Systematic error wavelength≤0.5 nm
Systematic temperature error
Wavelength Selection Increment1 nm
Wavelength range200 nm – 830 nm
Wavelength range absorptionScan (nm): 200 – 830 at 1 nm increments
dsDNA concentration range (UV 260 nm) µCuvette® G1.025 ng/µL – 1,500 ng/µL
dsDNA concentration range (UV 260 nm) UVette® 10 mm2.5 ng/µL – 150 ng/µL
dsDNA concentration range (UV 260 nm) UVette® 2 mm12.5 ng/µL – 750 ng/µL
dsDNA concentration range (for 1 to 10 mm path length)2.5 ng/µL – 1,500 ng/µL
Interfaces› USB master: for USB stick and thermal printer DPU-S445
› USB slave for connecting to a PC (All functions available without PC)
› Serial interface RS-232: for thermal printer DPU-414
› Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument
Power supply230 V, 50 – 60 Hz
Dimensions (W × D × H)295 × 400 × 150 mm / 11.6 × 15.7 × 6 in
Weight w/o accessories5.4 kg
Interval between two measuring points
Measuring time range
Adjustable temperature range

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