Absorption measuring range | 0 A – 3.0 A (260 nm) |
BSA concentration range (UV 280 nm) µCuvette® G1.0 | 758 ng/µL – 45,450 ng/µL |
BSA concentration range (UV 280 nm) UVette® 10 mm | 76 ng/µL – 4,545 ng/µL |
BSA concentration range (UV 280 nm) UVette® 2 mm | 379 ng/µL – 22,725 ng/µL |
BSA concentration range (for 1 to 10 mm path length, calculation factor for BSA = 1.515 µg/µL) | 76 ng/µL – 45,450 ng/µL (with fluorescence down to 0.01 ng/µL) |
Beam receiver fluorescence | Photodiodes |
Cuvette shaft | 12.5 mm × 12.5 mm |
Detector Type | CMOS photodiode array, 1024 pixel |
Emission wavelength I fluorescence | 520 nm, bandwidth: 15 nm |
Emission wavelength II fluorescence | 560 nm, bandwidth: 40 nm |
Excitation wavelength fluorescence | 470 nm, bandwidth: 25 nm |
Fluorescence measuring range | 0.5 nM – 2,000 nM fluorescein (emission wavelength 520 nm) |
Light source absorption | Xenon flash lamp |
Light source fluorescence | LED |
Measuring principle absorption | Single-beam absorption spectrophotometer with reference beam |
Measuring principle fluorescence | Confocal filter fluorometer with reference beam |
Method memory | >100 method programs |
Mode absorption | Spectrophotometer |
Mode fluorescence | Fluorometer |
Operator guidance language | Spanish, Italian, French, English, German, Japanese |
Power consumption | Approx. 15 W during operating step Approx. 5 W with dimmed display |
Random error absorption | ≤0.002 at A = 0 ≤0.005 (0.5 %) at A = 1 |
Random error fluorescence | ±2 % at 1 nM fluorescein |
Random error wavelength | ≤0.5 nm |
Random temperature error | – |
Sample light path length | 8.5 mm |
Scanning | yes |
Smallest step size | 1 nm |
Spectral bandwidth | ≤4 nm |
Systematic error absorption | – |
Systematic error wavelength | ±1 nm |
Systematic temperature error | – |
Wavelength Selection Increment | 1 nm |
Wavelength range | 200 nm – 830 nm |
Wavelength range absorption | Scan (nm): 200 – 830 at 1 nm increments |
dsDNA concentration range (UV 260 nm) µCuvette® G1.0 | 25 ng/µL – 1,500 ng/µL |
dsDNA concentration range (UV 260 nm) UVette® 10 mm | 2.5 ng/µL – 150 ng/µL |
dsDNA concentration range (UV 260 nm) UVette® 2 mm | 12.5 ng/µL – 750 ng/µL |
dsDNA concentration range (for 1 to 10 mm path length) | 2.5 ng/µL – 45,450 ng/µL (with fluorescence down to 1 pg/µL) |
Interfaces | › USB master: for USB stick and thermal printer DPU-S445 › USB slave for connecting to a PC (All functions available without PC) › Serial interface RS-232: for thermal printer DPU-414 › Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument |
Power supply | 230 V, 50 – 60 Hz |
Dimensions (W × D × H) | 295 × 400 × 150 mm / 11.6 × 15.7 × 6 in |
Weight w/o accessories | 5.4 kg |
Interval between two measuring points | – |
Measuring time range | – |
Adjustable temperature range | – |